abstract：AIM:To determine the Ca2+ source and cellular mechanisms of spontaneous Ca2+ oscillations in hippocampal astrocytes. METHODS:The cultured cells were loaded with Fluo-4 AM, the indicator of intracellular Ca2+, and the dynamic Ca2+ transients were visualized with confocal laser-scanning microscopy. RESULTS:The spontane...

journal_title：Acta pharmacologica Sinica

authors： Wang TF,Zhou C,Tang AH,Wang SQ,Chai Z

更新日期：2006-07-01 00:00:00

abstract：:A variety of Ca2+ signals, in the form of intercellular pulses and waves, have been reported to be associated with the various sequential stages of somitogenesis: from convergent extension and the formation of the paraxial mesoderm; during the patterning of the paraxial mesoderm to establish segmental units; throughou...

journal_title：Acta pharmacologica Sinica

authors： Webb SE,Miller AL

更新日期：2006-07-01 00:00:00

abstract：AIM:To investigate the effect of peroxisome proliferator-activated receptor gamma (PPAR gamma ) activation on the growth of rat hepatic stellate cells (HSC) and transforming growth factor beta (TGF-beta)-induced connective tissue growth factor (CTGF) expression. METHODS:After being treated with various amounts of the ...

journal_title：Acta pharmacologica Sinica

authors： Sun K,Wang Q,Huang XH

更新日期：2006-06-01 00:00:00

abstract：:Recepteur d'origine nantais (RON) belongs to a subfamily of receptor tyrosine kinases (RTK) with unique expression patterns and biological activities. RON is activated by a serum-derived growth factor macrophage stimulating protein (MSP). The RON gene transcription is essential for embryonic development and critical i...

journal_title：Acta pharmacologica Sinica

authors： Wang MH,Yao HP,Zhou YQ

更新日期：2006-06-01 00:00:00

abstract：AIM:To determine propofol concentration in the cerebral spinal fluid (CSF) of neurosurgical patients and carry out a preliminary population pharmacodynamic study. METHODS:Twenty-seven elective neurosurgical patients (12 men and 15 women) aged 17-74 years received propofol in a bolus dose of 2 mg/kg for 5 min and an in...

journal_title：Acta pharmacologica Sinica

authors： Li YH,Wu FS,Xu JG

更新日期：2006-05-01 00:00:00

abstract：AIM:To study the effect of adenovirus5-mediated human hepatocyte growth factor (Ad(5)-HGF) transfer on post-infarct heart failure in a swine model. METHODS:Twelve young Suzhong swine were randomly divided into 2 groups: the Ad(5)-HGF group (n=6) and the null-Ad(5) group (n=6). Four weeks after left anterior descending...

journal_title：Acta pharmacologica Sinica

authors： Wang W,Yang ZJ,Ma DC,Wang LS,Xu SL,Zhang YR,Cao KJ,Zhang FM,Ma WZ

更新日期：2006-05-01 00:00:00

abstract：AIM:To determine whether deguelin can regulate the expression of nuclear factor kappa B (NF-kappaB) binding protein (IkappaBalpha) in U937 human leukemia cells and Raji human B lymphoma cells. METHODS:The localization of IkappaBalpha protein was investigated by using an immunofluorescence method. The expression of Ika...

journal_title：Acta pharmacologica Sinica

authors： Chen WH,Chen Y,Cui GH

更新日期：2006-04-01 00:00:00

abstract：AIM:To investigate the modulatory effect of CJZ3, a lomerizine derivative, on P-glycoprotein (P-gp) function in rat brain microvessel endothelial cells (RBMEC). METHODS:RBMEC were isolated and cultured in Dulbecco modified Eagle medium/F12 (1:1) medium, and the amount of intracellular rhodamine 123 (Rh123) was determi...

journal_title：Acta pharmacologica Sinica

authors： Ji BS,He L,Li XQ,Liu GQ

更新日期：2006-04-01 00:00:00

abstract：AIM:To investigate the effect of incorporation of an isoleucine zipper (IZ) motif into CD40 on binding activity of CD40 for the CD40 ligand (CD40L). METHODS:Prokaryotic expression vectors for 2 soluble CD40 derivatives, shCD40His and shCD40IZ containing an IZ domain, were constructed and expressed in Escherichia coli....

journal_title：Acta pharmacologica Sinica

authors： He XH,Xu LH,Liu Y

更新日期：2006-03-01 00:00:00

abstract：:With the increasing incidence of drug-induced liver disease, attempts are being made to better understand the mechanisms behind these frequently life-endangering reactions. Analgesics and anti-inflammatory drugs are a major group exhibiting hepatotoxicity. We review research relating to these reactions, focusing on ul...

journal_title：Acta pharmacologica Sinica

authors： Manov I,Motanis H,Frumin I,Iancu TC

更新日期：2006-03-01 00:00:00

abstract：AIM:To investigate the ultrastructural localization of atrial natriuretic peptide (ANP)-synthesizing cells and the relationship between ANP-synthesizing cells and microvessels in rat gastric mucosa. METHODS:Immunohistochemistry techniques and postembedding immunoelectron microscopy techniques were used to validate the...

journal_title：Acta pharmacologica Sinica

authors： Li CH,Yang ZW,Yin ZR,Jin Z,Xing DG,Piao LH,Kim YC,Xu WX

更新日期：2006-02-01 00:00:00

abstract：:Alzheimer's disease (AD), a progressive degenerative disorder, is characterized by the presence of amyloid deposits, neurofibrillary tangles and neuron loss. Emerging evidence indicates that antioxidants could be useful either for the prevention or treatment of AD. It has been shown that melatonin is a potent antioxid...

journal_title：Acta pharmacologica Sinica

authors： Cheng Y,Feng Z,Zhang QZ,Zhang JT

更新日期：2006-02-01 00:00:00

abstract：AIM:Extract of Hominis Placenta (HP) has been used in oriental medicine as an agent for improving physiological function. The present study was conducted to investigate whether HP treatment in an experimental sciatic nerve injury animal model produces growth-promoting effects on regenerating peripheral nerve fibers aft...

journal_title：Acta pharmacologica Sinica

authors： Seo TB,Han IS,Yoon JH,Seol IC,Kim YS,Jo HK,An JJ,Hong KE,Seo YB,Kim DH,Park SK,Yang DC,Namgung U

更新日期：2006-01-01 00:00:00

abstract：AIM:To investigate the effect of cholecystokinin octapeptide (CCK-8) on the diacylglycerol-protein kinase C (DAG-PKC) signaling pathway in rat pulmonary interstitial macrophages (PIM) stimulated by lipopolysaccaride (LPS). METHODS:The PIM from rat lung tissues were isolated using the collagenase digestion method combi...

journal_title：Acta pharmacologica Sinica

authors： Xu SJ,Gao WJ,Cong B,Ma CL,Li SJ,Ling YL,Gu ZY,Yao YX

更新日期：2005-12-01 00:00:00

abstract：:Recently, two different chemicals have been matched as ligands with the same G-protein-coupled receptor (GPCR). Double-pairing of OGR1 family GPCRs with proton and lysolipid raises several questions. First, whether both are the real ligands for the GPCRs. Second, whether modulation of a GPCR by two chemicals could be ...

journal_title：Acta pharmacologica Sinica

authors： Im DS

更新日期：2005-12-01 00:00:00

abstract：AIM:To elucidate inhibition of protein kinase C alpha (PKC alpha) activity by staurosporine on apoptosis of oral cancer cell line tongue squamous cell carcinoma (TSCCa) cells and to clarify the role of survivin and caspase-3 in mediating apoptosis. METHODS:TSCCa cell viability was measured by MTT assay after 100 nmol/...

journal_title：Acta pharmacologica Sinica

authors： Zhang YX,Yu SB,Ou-Yang JP,Xia D,Wang M,Li JR

更新日期：2005-11-01 00:00:00

abstract：AIM:To investigate whether histidine can enhance the anticonvulsant efficacy of carbamazepine (CBZ) and simultaneously improve the spatial memory impairment induced by transauricular kindled seizures in Sprague-Dawley rats. METHODS:Chronic transauricular kindling was induced by repeated application of initially subcon...

journal_title：Acta pharmacologica Sinica

authors： Li Q,Jin CL,Xu LS,Zhu-Ge ZB,Yang LX,Liu LY,Chen Z

更新日期：2005-11-01 00:00:00

abstract：AIM:To study effects of arachidonic acid (AA) and its metabolites on the hyposmotic membrane stretch-induced increase in calcium-activated potassium currents (I(KCa)) in gastric myocytes. METHODS:Membrane currents were recorded by using a conventional whole cell patch-clamp technique in gastric myocytes isolated with ...

journal_title：Acta pharmacologica Sinica

authors： Yang M,Li XL,Xu HY,Sun JB,Mei B,Zheng HF,Piao LH,Xing DG,Li ZL,Xu WX

更新日期：2005-10-01 00:00:00

abstract：:AIDS is presently treatable, and patients can have a good prognosis due to the success of highly active antiretroviral therapy (HAART), but it is still not curable or preventable. High toxicity of HAART, and the emergence of drug resistance add to the imperative to continue research into new strategies and interventio...

journal_title：Acta pharmacologica Sinica

authors： Krambovitis E,Porichis F,Spandidos DA

更新日期：2005-10-01 00:00:00

abstract：AIM:To investigate effects of CpG oligodeoxynucleotide (CpG ODN) on the mRNA expression of transcription factors GATA binding protein 3 (GATA-3) and T-box expressed in T cells (T-bet) in asthmatic mice. METHODS:An asthmatic mouse model was established and treated with CpG ODN. Total inflammatory cells and eosinophils ...

journal_title：Acta pharmacologica Sinica

authors： Wu ZQ,Xu YP,Xiang H,Shen HH

更新日期：2005-09-01 00:00:00

abstract：AIM:To investigate the blood pressure (BP), baroreflex sensitivity (BRS), and organ damage in hybrids of spontaneously hypertensive rats and Sprague-Dawley rats. METHODS:Spontaneously hypertensive rats and Sprague-Dawley rats were crossbred, and the F1 hybrids were inbred randomly to produce an F2 generation. At the a...

journal_title：Acta pharmacologica Sinica

authors： Xie HH,Shen FM,Miao CY,Su DF

更新日期：2005-09-01 00:00:00

abstract：AIM:To investigate the mitochondria-initiated apoptosis pathway involved in Carbon tetrachloride (CCl4) hepatotoxicity in vitro. METHODS:Several cytotoxicity endpoints, including WST-8 metabolism, lactate dehydrogenase leakage and morphological changes, were examined. The 5,5'-dithio-bis(2-nitrobenzoic acid) reaction ...

journal_title：Acta pharmacologica Sinica

authors： Cai Y,Gong LK,Qi XM,Li XH,Ren J

更新日期：2005-08-01 00:00:00

abstract：AIM:To study the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines. METHODS:The experimental model of AS in rats were established by intraperitioneal injection of vit...

journal_title：Acta pharmacologica Sinica

authors： Jiao YB,Rui YC,Li TJ,Yang PY,Qiu Y

更新日期：2005-07-01 00:00:00

abstract：AIM:To investigate the influence of picroside II on hepatocyte apoptosis and its mechanism. METHODS:Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-q...

journal_title：Acta pharmacologica Sinica

authors： Gao H,Zhou YW

更新日期：2005-06-01 00:00:00

abstract：:Specific chromosome aberrations are frequently detected during the development of hepatocellular carcinoma. Molecular cytogenetic approaches such as comparative genomic hybridization and loss of heterozygosity analyses have provided fruitful information on changes in HCC cases at the genomic level. Mapping of chromoso...

journal_title：Acta pharmacologica Sinica

authors： Lau SH,Guan XY

更新日期：2005-06-01 00:00:00

abstract：AIM:To determine whether mycophenolate mofetil (MMF) has beneficial effects on refractory idiopathic thrombocytopenic purpura (ITP) and the corresponding cellular mechanism. METHODS:Twenty refractory ITP patients resistant to corticosteroid and/or splenectomy and chemical therapy were given MMF 1.5-2.0 g/d orally for ...

journal_title：Acta pharmacologica Sinica

authors： Zhang WG,Ji L,Cao XM,Chen YX,He AL,Liu J,Zhao WH,Zou SP

更新日期：2005-05-01 00:00:00

abstract：AIM:To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (I(HVA)) and its modulation by ginkgolide B (Gin B). METHODS:The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats. RESULTS:Al 0.1 mmol/L (low concentration)...

journal_title：Acta pharmacologica Sinica

authors： Chen L,Liu CJ,Tang M,Li A,Hu XW,Du YM,Shen JJ,Lu YL,Heschler J

更新日期：2005-05-01 00:00:00

abstract：AIM:To investigate the effect of 7-hydroxystaurosporine (UCN-01), a selective protein kinase C (PKC) inhibitor, on cell growth, migration, and invasion in invasive human glioblastoma U-87MG cells. METHODS:PKC activity was determined based on the PKC-catalyzed transfer of the (32)P-phosphate group from [g-(32)P]ATP int...

journal_title：Acta pharmacologica Sinica

authors： Meng QH,Zhou LX,Luo JL,Cao JP,Tong J,Fan SJ

更新日期：2005-04-01 00:00:00

abstract：AIM:To determine the role of dietary low histamine on the seizure development of pentylenetetrazol (PTZ)-induced kindling in rats. METHODS:After 14 d of feeding on a low histamine diet (LH, containing 0.145 mumol/g of histamine), the rats were chemically kindled by repeated intraperitoneal injection of a subconvulsant...

journal_title：Acta pharmacologica Sinica

authors： Jin CL,Sakurai E,Kiso Y,Luo JH,Yanai K,Chen Z

更新日期：2005-04-01 00:00:00

abstract：AIM:To investigate the mechanism of action of a potent analgesic, (+/-)-meptazinol. METHODS:The structures of meptazinol enantiomers were compared with opioid pharmacophore and tramadol. RESULTS:Neither enantiomer of meptazinol fitted any patterns among the opioid pharmacophore and tramadol, although they did share s...

journal_title：Acta pharmacologica Sinica

authors： Li W,Hao JL,Tang Y,Chen Y,Qiu ZB

更新日期：2005-03-01 00:00:00

abstract：:Wound healing impairment represents a particularly challenging clinical problem to which no efficacious treatment regimens currently exist. The factors ensuring appropriate intercellular communication during wound repair are not completely understood. Although protein-type mediators are well-established players in thi...

journal_title：Acta pharmacologica Sinica

authors： Luo JD,Chen AF

更新日期：2005-03-01 00:00:00

abstract：AIM:To investigate the effects of onychin on the proliferation of cultured rat artery vascular smooth muscle cells (VSMCs) in the presence of 10% new-born calf serum (NCS). METHODS:Rat VSMCs were incubated with onychin 150 micromol/L or genistein 10 micromol/L in the presence of 10% NCS for 24 h. The proliferation of ...

journal_title：Acta pharmacologica Sinica

authors： Yang M,Huang HL,Zhu BY,Tuo QH,Liao DF

更新日期：2005-02-01 00:00:00

abstract：AIM:To study the effects of the caspase-1 inhibitor Ac-YVAD-CHO on quinolinic acid (QA)-induced apoptosis. METHODS:Rats were pre-treated with intrastriatal infusion of Ac-YVAD-CHO (2-8 microg) before intrastriatal injection of QA (60 nmol). Striatal total proteins, genomic DNA, and nuclear proteins were isolated. The ...

journal_title：Acta pharmacologica Sinica

authors： Cao Y,Gu ZL,Lin F,Han R,Qin ZH

更新日期：2005-02-01 00:00:00

abstract：AIM:To investigate the effects of pravastatin, a potent 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, on impaired endothelium-dependent relaxation induced by lysophosphatidylcholine (LPC), the major component of oxidized low-density lipoprotein, in rat thoracic aorta. METHODS:Both the endotheliu...

journal_title：Acta pharmacologica Sinica

authors： Deng HF,Xiong Y

更新日期：2005-01-01 00:00:00

abstract：AIM:To construct an HEK293 cell line stably expressing human dopamine D1 receptor (D1R). METHODS:cDNA was amplified by RT-PCR using total RNA from human embryo brain tissue as the template. The PCR products were subcloned into the plasmid pcDNA3 and cloned into the plasmid pcDNA3.1. The cloned D1R cDNA was sequenced a...

journal_title：Acta pharmacologica Sinica

authors： Sun WC,Jin L,Cao Y,Wang LZ,Meng F,Zhu XZ

更新日期：2005-01-01 00:00:00

abstract：AIM:To model the three-dimensional structure and investigate the interaction mechanism of the proprotein convertase furin/kexin and their inhibitors (eglin c mutants). METHODS:The three-dimensional complex structures of furin/kexin with its inhibitors, eglin c mutants, were generated by modeller program using the newl...

journal_title：Acta pharmacologica Sinica

authors： Cai XH,Zhang Q,Ding DF

更新日期：2004-12-01 00:00:00

abstract：AIM:To explore whether the differential effects of Zn2+ on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn2+. METHODS:[3H]ryanodine binding assays were performed to examine the effect of Zn2+ on ryanodine binding to the SR in the...

journal_title：Acta pharmacologica Sinica

authors： Xie H,Chen KY,Zhu PH

更新日期：2004-12-01 00:00:00

abstract：AIM:Using sparse data of valproate (VPA) serum concentrations to build a population pharmacokinetic (PPK) model of VPA in Chinese children with epilepsy and to predict serum concentrations for new patients using a Bayesian approach. METHODS:Two hundred epileptic children, whose VPA serum concentrations were collected,...

journal_title：Acta pharmacologica Sinica

authors： Jiang DC,Wang L

更新日期：2004-12-01 00:00:00

abstract：AIM:To find novel proteins that may bind to alpha1A-adrenergic receptor (alpha1A-AR) and investigate their interactions with the other two alpha1-AR subtypes (alpha1B-AR and alpha1D-AR) with an expectation to provide new leads for the function study of the receptors. METHODS:Yeast two-hybrid assay was performed to scr...

journal_title：Acta pharmacologica Sinica

authors： Zhang T,Xu Q,Chen FR,Han QD,Zhang YY

更新日期：2004-11-01 00:00:00

abstract：AIM:To explore the action mechanism of antidepressants. METHODS:The PC12 cell proliferation was detected by flow cytometry. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS:Treatment with N-methylaspartate (NMDA) 600...

journal_title：Acta pharmacologica Sinica

authors： Li YF,Zhang YZ,Liu YQ,Wang HL,Yuan L,Luo ZP

更新日期：2004-11-01 00:00:00